Measurement of Cell Death in Mammalian Cells

Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways death; (2) method to measure using lactate dehydrogenase (LDH) release as marker membrane integrity; (3) flow cytometry that simultaneously measures two types death, necrosis apoptosis; (4) use fluorescence microscopy nuclear morphology assess apoptosis necrosis; (5) the multi-well plates high-content analysis imaging systems morphology; (6) discussion assays determine mechanisms death. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Measurement plasma integrity viability LDH 2: 3: Determination Alternate Assessment DAPI PI 4: time-dependent toxicity markers